The Robert P. Apkarian Integrated Electron Microscopy Core (IEMC) at Emory University provides electron microscopy (EM) training and services to academic, clinical, and industry users. The IEMC is located at two sites on the Emory University main campus. The Cherry L. Emerson Hall site provides services and training that include conventional (room temperature) transmission electron microscopy (TEM), cryo-transmission electron microscopy (cryo-TEM), cryo-electron tomography (cryo-ET), conventional scanning electron microscopy (SEM), cryo- and room temperature focused ion beam – scanning electron microscopy (FIB-SEM), cryo- scanning electron microscopy (cryo-SEM), energy dispersive spectroscopy (EDS), cryo-electron microscopy of vitrified sections (CEMOVIS), and microcrystal electron diffraction (micro-ED). The Biochemistry Connector site provides services and training in TEM, single particle cryo-TEM (sp-cryo-TEM), and cryo-ET. Instrumentation at the IEMC includes a ThermoFisher Talos Arctica 200 kV field emission TEM (equipped with a Gatan Image Filter and K3 direct electron detector), a JEOL JEM-2200FS 200 kV field emission TEM (equipped with an in-column Omega filter, hole-free phase-plates, a Direct Electron DE-20, and a Gatan K2 direct electron detectors), a ThermoFisher Talos 120 kV TEM, a JEOL JEM-1400 120 kV TEM, a Hitachi HT7700 120 kV TEM, a JEOL JSM-IT700HR field emission SEM with EDS capability, a ThermoFisher Hydra Bio Plasma FIB-SEM (with an integrated Delmic Meteor Fluorescent Microscope (iFLM)), and a Leica DM6 FS (with STP8000) cryo-CLEM microscope. Additional sample preparation equipment includes two ThermoFisher Vitrobot Mark IV (one on each IEMC site), a Gatan CP3, several plasma-cleaners and glow dischargers, carbon/gold evaporators and sputter coaters, three room temperature ultramicrotomes, a Leica UC6i/FC6 cryo-ultramicrotome, a Baltec HPM-010 high-pressure freezer, and a Leica EM AFS2 instrument for freeze substitution and progressive lowering of temperature (PLT) techniques.

There are two dedicated workstations for image processing, cryo-TEM and cryo-ET data reconstruction and refinement, and data storage. A dedicated NAS Synology system is maintained for data back up and transfer using Globus.

If you used services provided by the Robert P. Apkarian Integrated Electron Microscopy Core, please include the following text in the Acknowledgments section of your publications and submit a PDF of your publication for our records:

This study was supported by Emory University Robert P. Apkarian Integrated Electron Microscopy Core Facility (RRID: SCR_023537), which is subsidized by the School of Medicine and Emory College of Arts and Sciences.

For more infirmation contact Rocardo Guerrero-Ferreira at ricardo.guerrero@emory.edu