Emory Transgenic and Gene Targeting Core - MAJOR EQUIPMENT
Last Updated: July 01, 2022

Updated: 1 July 2022

 

Specific Fields Relevant for TMF Users

 

Emory Mouse Transgenic and Gene Targeting Core (TMF): The Emory Mouse Transgenic and Gene Targeting Core provides state of the art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, or cryopreserving existing strains of mice. With laboratories and animal facilities located on the ground floor of the Health Sciences Research Building (HSRB), the TMF provides rodent-related services that are supported by the following infrastructure.

 

 

Microinjection/Gene Editing (EG42)

 

Microinjection Station #1:

 

Leica DMi8 Inverted microscope with Phase contrast and DIC optics

 

Narishige manual micromanipulators

 

Eppendorf microinjectors

 

Eppendorf FemtoJet 4i transjector

 

Prime Tech PMM4G Piezo Impact Drive

 

Brook Industries heating/cooling Thermal Stage System

 

 

 

Microinjection Station #2:

 

Leica DMi8 Inverted microscope with Phase contrast and DIC optics

 

Narishige manual micromanipulators

 

Eppendorf microinjectors

 

Eppendorf FemtoJet 4i transjector

 

Brook Industries heating/cooling Thermal Stage System

 

 

 

Embryo collection, manipulation, and rederivation (EG42)

 

Leica M80 Binocular Stereo Microscope with heating stage (x2)

 

Leica M60 Binocular Stereo Microscope

 

Panasonic CO2 Incubator (x2)

 

Sutter P-87 Micropipette puller

 

BTX Electroporation system

 

             

 

ES Cell Culture (EG42)

 

Nuaire Class II Biological Safety Cabinet

 

ThermoScientific centrifuge

 

Eppendorf Centrifuge

 

Panasonic CO2 Incubator

 

Leica DMi1 Inverted Microscope

 

BioRad Electroporation Unit

 

             

 

Sperm/Embryo Cryopreservation (EG41)

 

FTS Systems Bio-Cool IV

 

Locator 4 Liquid Nitrogen Dewar

 

MVE Liquid Nitrogen Dewars (x9)

 

CEROS-2 Animal Sperm Analyzer

 

 

 

Molecular Biology (EG41)

 

Ohaus Explorer Analytical Balance

 

Beckman-Coulter Allegra 64R high-speed Centrifuge

 

ABI 9700 thermal cycler (x2)

 

Thermo Scientific NanoDrop Spectrophotometer            

 

Nuaire Class II Biological Safety Cabinet

 

Fisher Marathon 16KM Centrifuge

 

Analytik Jena VisionWorks gel imaging system

 

 

 

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