Updated: April 2023
Specific Fields Relevant for TMF Users
Emory Mouse Transgenic and Gene Targeting Core (TMF): The Emory Mouse Transgenic and Gene Targeting Core provides state of the art equipment and expertise for making genetically engineered mouse models, moving alleles on or off campus, or cryopreserving existing strains of mice. With laboratories and animal facilities located on the ground floor of the Health Sciences Research Building (HSRB), the TMF provides rodent-related services that are supported by the following infrastructure.
Microinjection/Gene Editing (EG42)
Microinjection Station #1:
Leica DMi8 Inverted microscope with Phase contrast and DIC optics
Narishige manual micromanipulators
Eppendorf microinjectors
Eppendorf FemtoJet 4i transjector
Prime Tech PMM4G Piezo Impact Drive
Brook Industries heating/cooling Thermal Stage System
Microinjection Station #2:
Leica DMi8 Inverted microscope with Phase contrast and DIC optics
Narishige manual micromanipulators
Eppendorf microinjectors
Eppendorf FemtoJet 4i transjector
Brook Industries heating/cooling Thermal Stage System
Embryo collection, manipulation, and rederivation (EG42)
Leica M80 Binocular Stereo Microscope with heating stage (x2)
Leica M60 Binocular Stereo Microscope
Panasonic CO2 Incubator (x2)
Sutter P-87 Micropipette puller
BTX Electroporation system
ES Cell Culture (EG42)
Nuaire Class II Biological Safety Cabinet
ThermoScientific centrifuge
Eppendorf Centrifuge
Panasonic CO2 Incubator
Leica DMi1 Inverted Microscope
BioRad Electroporation Unit
Sperm/Embryo Cryopreservation (EG41)
FTS Systems Bio-Cool IV
Locator 4 Liquid Nitrogen Dewar
MVE Liquid Nitrogen Dewars (x9)
CEROS-2 Animal Sperm Analyzer
Molecular Biology (EG41)
Ohaus Explorer Analytical Balance
Beckman-Coulter Allegra 64R high-speed Centrifuge
ABI 9700 thermal cycler (x2)
Thermo Scientific NanoDrop Spectrophotometer
Nuaire Class II Biological Safety Cabinet
Fisher Marathon 16KM Centrifuge
Analytik Jena VisionWorks gel imaging system